Extremely Comprehensive Stool Testing – More than pathogen detection

We live in interesting times. New technologies allow for laboratory identification of microbial species that were previously unavailable or unidentifiable. With the advent of newer technologies, where does that leave the old, reliable laboratory standards?

Right on center stage, where they belong. Current DNA technologies, using validated platforms (now used for rapid identification in major medical institutions) may identify known pathogens but cannot provide information about antibiotic sensitivities. a gene present in the microbiome is not necessarily actively expressed. Nor do advances in microbial identification provide information about a patient’s digestive status, immune status, tissue inflammation, etc. Many types of information are required to properly assess a symptomatic patient, and pathogen detection is only one piece of a much larger puzzle.

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References

Korecka, Agata and Arulampalam, Velmurugesan. The gut microbiome: scourge, sentinel or spectator? Journal of Oral Microbiology. 2012, vol. 4: 9367.

Laboratory Detection of Intestinal Dysbiosis. Schreckenberger, Paul PhD. St. Charles : Doctor’s Data.

The Standards of Practice in Clinical Laboratory Testing: Microbiology, Mycology and Susceptibility Testing. Quig, David PhD. s.l. : Doctor’s Data.

Papoff, Paola et al. gut Microbial Translocation in Critically Ill Children and effects of Supplementation with pre- and pro Biotics. International Journal of Microbiology. [Online] august 15, 2012. [Cited: april 30, 2013.] https://www.ncbi.nlm.nih.gov/pmc/ articles/pMC3426218/.

Klindworth, Anna et al. evaluation of general 16S ribosomal RNa gene pCR primers for classical and next-generation sequencingbased diversity studies. Nucleic Acids Research. [Online] July 31, 2012. [Cited: april 30, 2013.] https://nar.oxfordjournals.org/content/ early/2012/08/27/nar.gks808.long.

Wolk, Donna M. and Dunne, W. Michael Jr. New Technologies in Clinical Microbiology. Journal of Clinical Microbiology. [Online] September 2011. [Cited: april 19, 2013.] https://jcm.asm.org/content/49/9_ Supplement/S62.full.

Harris, Kathryn A. and Hartley, John C. Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service. Journal of Medical Microbiology. [Online] april 19, 2003. [Cited: april 19, 2013.] https://jmm.sgmjournals.org/ content/52/8/685.full.

Ndao, Momar. Diagnosis of parasitic Diseases: Old and New approaches. Interdisciplinary Perspectives on Infectious Diseases. [Online] august 29, 2009. [Cited: april 19, 2013.] https://www.hindawi.com/ journals/ipid/2009/278246/.

Pyle, Gail, G., et al. Low-Dose gluten Challenge in Celiac Sprue: Malabsorptive and antibody Responses. Clincal Gastroenterology and Hepatology. [Online] July 2005. [Cited: april 19, 2013.] https://www.cghjournal.org/ article/S1542-3565(05)00365-4/abstract.

Health Grades Inc. Bowel Inflammation. www.RightDiagnosis.com. [Online] March 01, 2013. [Cited: april 19, 2013.] https:// www.rightdiagnosis.com/sym/bowel_ inflammation.htm.

Mucus in Stool. www.RightDiagnosis.com. [Online] March 01, 2013. [Cited: april 19, 2013.] https://www.rightdiagnosis.com/ symptoms/mucus_stool/causes.htm. Latiff, Amir H. Abdul and Kerr, Michael A. The clinical significance of immunoglobulin
a deficiency. Annals of Clinical Biochemistry. [Online] March 01, 2007. [Cited: april 22, 2013.] https://acb.rsmjournals.com/ content/44/2/131.short.

Wong, Julia M. W. RD, et al. Colonic Health: Fermentation and Short Chain Fatty acids. Journal of Clinical Gastroenterology. [Online]
March 2006. [Cited: april 30, 2013.] https:// journals.lww.com/jcge/abstract/2006/03000/ Colonic_Health__Fermentation_and_Short_ Chain_Fatty.15.aspx.

WebMD. Blood in Stool. www.webMD.com. [Online] april 25, 2013. [Cited: May 03, 2013.] https://www.webmd.com/digestive-disorders/ blood-in-stool?page=2.

Healthwise staff. Stool analysis. www. webMD.com. [Online] October 13, 2010. [Cited: May 03, 2013.] https://www.webmd. com/digestive-disorders/stool-analysis. Picco, Michael F. MD. Stool color: When to worry. www.mayoclinic.com. [Online] October 12, 2012. [Cited: May 03, 2013.] https://www. mayoclinic.com/health/stool-color/aN00772.

Lawrence Berkley National Laboratory. CSI: Microbial version without pCR. https://newscenter.lbl.gov/. [Online] U.S. Department of energy, September 11, 2011. [Cited: april 19, 2013.] https://newscenter.lbl.gov/featurestories/ 2011/09/21/pcr-free-microbialanalysis/.

Farrance PhD, Christine. Compliance and Microbial Identification. https://www. naturalproductsinsider.com/. [Online] Natural products Insider, august 20, 2012. [Cited: april 19, 2013.] https://www.naturalproductsinsider.com/articles/2012/08/compliance-and-microbial-identification.aspx.

 

1 reply
  1. Dr Ashley Berry says:

    While I agree that bright red in the stools may be haemorrhoids, bowel cancer must be excluded by a colonoscopy. This applies to all adults, even those under 40 years of age.

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